Tg mpeg1:mcherry

Author: djmx

August undefined, 2024

Web20 Jun 2024 · We first assessed macrophage heterogeneity and recruitment dynamics following larval cardiac injury. We crossed the zebrafish pan-macrophage reporter line Tg(mpeg1:GFP) with Tg(csf1ra:gal4;UAS:mCherry-NfsB) (shortened here to csf1ra: mCherry) (Figure S1 A, related to Figure 1).Csf1ra (colony stimulated factor 1 receptor) is … Web30 Jul 2024 · Histological staining of isolated wt1b:eGFP +;mpeg1:mCherry + and mpeg1:mCherry + cells from cryoinjured hearts revealed a typical myeloid cell shape with large cytoplasmic areas and an irregular nuclear shape. Double-positive cells were slightly larger than mpeg1:mCherry + cells (Figures 1J and 1K), suggesting that wt1b:eGFP …

mpeg1 promoter transgenes direct macrophage-lineage …

Web10 Aug 2024 · mpeg1.1 + lymphoid-like cells are found in the spleen, blood, and caudal fin of adult Tg ( mpeg1.1:mCherry ); TgBAC ( csf1ra:GFP) zebrafish. ( A – C) Confocal imaging in the spleen (A), circulation (B), and caudal fin (C). Arrowheads identify mpeg1.1+ cells. (A and C) Whole-mount imaging in fixed tissues. WebThe same phenomenon was also found in Tg(coro1a:DsRed;mpeg1:GFP) double transgenic lines [37] ( Figure S2A). Through fluorescence co-localization analysis of DsRed + and … duluth minnesota weather summer

Identification of polarized macrophage subsets in …

Web35 rows · Transgenic Construct Tg (mpeg1:mCherry) ID ZDB-TGCONSTRCT-120117-2 … Web21 Apr 2024 · In this chapter, we provide a detailed description of how to induce wounds in zebrafish double transgenic Tg(mpeg1:mCherry); TgBAC(tnfa:GFP) larvae [9, 12], which mark macrophages in red and pro-inflammatory cells in green. Next, we describe how to dissociate larval tissue in a manner optimized for immune cells, and how to best FACS … Web20 Nov 2024 · Homozygous mutants were outcrossed to the Tg (mpeg1:mCherry-F);TgBAC (mpx: EGFP) double transgenic line [88, 89], and the offspring with GFP and mCherry fluorescence were subsequently incrossed to produce the Tg (mpeg1:mCherry-F);TgBAC (mpx: EGFP) line. Bacterial strain preparation. duluth minnesota to grand marais

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Zebrafish larvae as experimental model to expedite the search for …

Web25 Feb 2024 · Transgenic Tg(mpeg1:mCherry) zebrafish embryos stably express mCherry (magenta) within all macrophages. b,c) Whole embryo (10× magnification) and tissue level (40× magnification) views of srLNP biodistribution and eGFP expression within the embryonic zebrafish at 1.5 hpi. At this timepoint, srLNPs were mainly associated with … WebFigure Legend Snippet: ( A ) Maximum projection of a microglia (green) making contact with myelin sheaths (magenta) in a 4 dpf Tg(mpeg1:EGFP; sox10:mRFP) zebrafish spinal cord. ( B-F ) Microglia scanning behavior along myelin sheaths in the spinal cord was analyzed by confocal time-lapse imaging of 3-4 dpf Tg(mpeg1:EGFP; mbp:mCherry-CAAX) or … duluth minn hockey scoreWebFirst, by performing L-plastin (Lcp1) immunohistochemistry and using transgenic Tg(mpeg1.1:GFP) or Tg(mpeg1.1:mCherry) fish, we analyzed the distribution of … duluth minnesota home show

"Web24 Aug 2024 · The compound transgenic line Tg(actc1b:GFP); Tg( mpeg1 :GAL4FF/ UAS :NfsB-mCherry), in which differentiated muscle expresses GFP and macrophages are labelled by mCherry fluorescence, allowed the wound region to be defined while " - Tg mpeg1:mcherry

Tg mpeg1:mcherry

Distinct regulatory networks control the development of …

Web8 Apr 2024 · Fig. 1. In moonshine mutant zebrafish, primitive macrophages prematurely disappear between 54 hpf and 4 dpf (A) mCherry+ macrophages in live 4 dpf Tg(mpeg1:mCherry-F) control sibling (top) and mon NQ039 mutant (bottom) larvae; maximum projection, lateral view. (B) Quantification of mCherry+ macrophages in live … Web7 Jan 2024 · Mpeg1.1 expression does not discriminate a subset of phagocytic B cells in zebrafish. (A) Distribution of fluorescence in cell suspension from the IPEX of adult …

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Webated a reporter line Tg(mpeg1:loxP-DsRedx-loxP-GFP), in which macrophages (Ellett et al., 2011) were DsRedx+ under normal conditions but switched to GFP+ upon Cre/loxP-medi-ated recombination. The reporter line was outcrossed with Tg(hsp70:mCherry-T2a-CreERT2) ﬁsh to generate double trans-genic line, in which IR-LEGO-CreER-loxP labeling ... Web10 Jun 2024 · A similar 10 mM short treatment regimen using Tg (mpx:KalTA4;UAS:nfsB-mCherry) larvae resulted in 4.9% ablation ( Fig. 1J and Supplementary Data ). In summary, here we present the Tg (lyz:YFP-NTR2.0) transgenic line as a new tool for fast and effective ablation of neutrophils in zebrafish. This line can achieve complete loss of neutrophils ...

Web26 Jan 2024 · To prove this is indeed the case, we outcrossed pu.1 ∆839 with Tg(hsp70:mCherry-T2a-CreER T2;mpeg1:loxP-DsRedx-loxP-eGFP) (we herein refer to loxP-DsRedx-loxP-eGFP as LRLG) reporter line, in which CreER recombinase is induced by spatial-restricted infrared light–mediated heat shock and subsequently activated by tamoxifen, … Web4 Aug 2024 · Next, we analyzed double transgenic cd45:CFP‐NTR;runx1:mCherry embryos at 48 and 72 hpf by flow cytometry, and triple transgenic cd45:CFP‐NTR;runx1:mCherry;mpeg1:GFP embryos at 60 hpf by live imaging (Appendix Fig S3A–C). Our data clearly show that CFP and mCherry never overlap, meaning that the 7.6 …

Web10 Aug 2024 · macrophages from the early stage of regeneration, Tg(mpeg1:mCherry-F) were injected with L-clodronate (or L-clo) or L-PBS (control) at 48 hpf and fin were …

WebZebrafish strains AB, Tg(mpx:GFP) and Tg(mpeg1:mCherry) are raised and maintained using standard laboratory procedures as described . Embryos are obtained via natural mating and cultured in embryo E2 buffer in 28 ± 0.5 °C incubator. All experiments in this study are conducted according to the ethical guidelines established by the St. Michael ...

WebTg (mpeg1:mCherryCAAX) ( 1) Type engineered_plasmid Regulatory Regions mpeg1.1 ( 1) Coding Sequences CAAX ( 1) mCherry ( 1) [Em λ] [Ex λ] 610 (red) 587 (red) … duluth minn tv stationsWebTo confirm this point, the transgenic ZF line Tg (mpeg1:mCherry) was previously exploited to visualize the interactions between macrophages and other immune cells or pathogens in vivo and to reexamine macrophage roles in inflammation, wound healing, and development, as well as their interactions with other cell types (e.g., vasculature, muscle) in vivo ; more … duluth minn. weatherWeb18 Nov 2024 · To label most RBI- or AGM-derived macrophages, we heat-shocked the entire respective region in Tg(hsp70:mCherry-T2a-CreERT2;mpeg1:loxP-DsRedx-loxP-GFP) double transgenic embryos and analyzed at 1 month . We found that while both RBI and AGM irradiation labeled widely distributed amoeboid cells, only AGM ... duluth minnesota to superior wiWebVentricles of wildtype Tg ( mpeg1:mCherry) were collected into PBS containing 10 mM HEPES, 30 mM taurine, and 5.5 mM glucose and cells dissociated by the addition of 0.25% trypsin, 12.5 M CaCl 2, and 5 mg/mL Collagenase II … duluth minnesota to canadaWebTg (mpeg1:mCherryCAAX) Type engineered_plasmid Regulatory Regions mpeg1.1 ( 1) Coding Sequences mCherry ( 1) [Em λ] [Ex λ] 610 (red) 587 (red) Fpbase:mCherry … duluth minn live camsWeb10 Feb 2024 · In brief, 4 dpf Tg(mpeg1:mCherry) larvae were subject to needle-stab injury and the injured region was dissected at 1, 2 and 3 dpi and tissue dissociated into a single … duluth minnesota waterfront homes for saleWeb17 Jul 2024 · NADPH oxidase inhibitor Apocynin increases susceptibility to notochord infection. (A-B) E. coli- GFP were injected in the notochord of 2 dpf tg (mpx: GFP) embryos in DMSO or Apocynin treatment conditions. (A) At 1 dpi, superoxide production was visualised using DHE (red), neutrophils and E. coli were detected using GFP. duluth minn weather radar